merck millipore,默克密理愽
  • merck millipore,默克密理博,05-1076,Anti-c-Jun Antibody, clone 6E4.4

  • merck millipore,默克密理博,05-1076,Anti-c-Jun Antibody, clone 6E4.4

    产品名称：Anti-c-Jun Antibody, clone 6E4.4
    产品型号：05-1076
    Anti-c-Jun Antibody, clone 6E4.4 is a Mouse Monoclonal Antibody for detection of c-Jun also known as JNK1 alpha protein kinase, JNK1 beta protein kinase & has been validated in WB, ICC.

    merck millipore,默克密理博,05-1076,Anti-c-Jun Antibody, clone 6E4.4

  • 产品介绍
  • merck millipore,默克密理博,05-1076,Anti-c-Jun Antibody, clone 6E4.4

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, H, MICC, WBMPurifiedMonoclonal Antibody
    描述
    产品目录编号 05-1076
    Replaces 04-210
    描述 Anti-c-Jun Antibody, clone 6E4.4
    Alternate Names
    • protein kinase JNK1
    • JNK1 alpha protein kinase
    • JNK1 beta protein kinase
    • JUN N-terminal kinase
    • Stress-activated protein kinase JNK1
    • c-Jun N-terminal kinase 1
    • mitogen-activated protein kinase 8
    • mitogen-activated protein kinase 8 isoform JNK1 alpha1
    • mitogen-activated protein kinase 8 isoform JNK1 beta2
    背景信息 c-Jun is a component of the transcription factor AP-1 that binds and activates transcription at TRE/AP-1 elements and appears to be a major downstream target of the SAPK/JNK signaling pathway. The transcriptional activity of c-Jun is regulated by phosphorylation at Ser63 and Ser73. Extracellular signals including growth factors, transforming oncoproteins and UV irradiation stimulate phosphorylation of c-Jun at Ser63/73 and activate c-Jun dependent transcription. Mutation of Ser63/73 renders c-Jun nonresponsive to mitogenic and stress induced signaling pathways. The MAP kinase homologue, SAPK/JNK, binds to the N-terminal region of c-Jun and phosphorylates c-Jun at Ser63/73. In addition, the activity of SAPK/JNK is stimulated by the same signals that activate c-Jun.
    产品信息
    格式 Purified
    控制
    • NIH/3T3 cell lysate
    演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH7.4), 150 mM NaCl with 0.05% sodium azide.
    应用
    应用 Anti-c-Jun Antibody, clone 6E4.4 is a Mouse Monoclonal Antibody for detection of c-Jun also known as JNK1 alpha protein kinase, JNK1 beta protein kinase & has been validated in WB, ICC.
    主要应用
    • Immunocytochemistry
    • Western Blotting
    应用说明 Western Blot Analysis (SNAP ID Technique): 5 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate using the SNAP ID Tecnique).

    Immunocytochemistry Analysis: Confocal fluorescent analysis of HeLa, C2C12, and A431 cells using anti-c-Jun, clone 6E4.4 mouse monoclonal antibody (see page 2).
    生物信息
    免疫原品种 GST-tagged recombinant protein corresponding to c-Jun.
    克隆 6E4.4
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 This antibody detects c-Jun, regardless of phosphorylation state.
    同种型 IgG1κ
    品种反应性 RatHumanMouse
    Species Reactivity Note Demonstrated to react with human, mouse and rat.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NP_002219.1
    Entrez基因汇总 This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involvedin both translocations and deletions in human malignancies.
    基因符号
    • SAPK1
    • EC 2.7.11.24
    • JNK
    • JNK-46
    • JNK1
    • JNK1A2
    • JNK21B1/2
    • OTTHUMP00000019552
    • OTTHUMP00000019555
    • OTTHUMP00000019556
    • OTTHUMP00000019558
    • PRKM8
    纯化方法 Protein G purfied
    UniProt编号
    • P45983
    UniProt汇总 FUNCTION: Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4).

    JNK1 isoforms display different binding patterns: beta-1 preferentially binds to c-Jun, whereas alpha-1, alpha-2, and beta-2 have a similar low level of binding to both c-Jun or ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms.

    CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

    COFACTOR: Magnesium.

    ENZYME REGULATION: Activated by threonine and tyrosine phosphorylation by either of two dual specificity kinases, MAP2K4 and MAP2K7. Inhibited by dual specificity phosphatases, such as DUSP1.

    SUBUNIT STRUCTURE: Binds to at least four scaffolding proteins, MAPK8IP1/JIP-1, MAPK8IP2/JIP-2, MAPK8IP3/JIP-3/JSAP1 and SPAG9/MAPK8IP4/JIP-4. These proteins also bind other components of the JNK signaling pathway. Interacts with TP53 and WWOX. Interacts with JAMP. Forms a complex with MAPK8IP1 and RGNEF By similarity. Interacts with NFATC4.

    DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    PTM: Dually phosphorylated on Thr-183 and Tyr-185, which activates the enzyme.

    SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

    Contains 1 protein kinase domain.
    产品使用声明
    质量保证 Evaluated by Western Blot in NIH/3T3 cell lysate.

    Western Blot Analysis: 0.5-2 µg/mL of this antibody detected c-Jun on 15 µg of NIH/3T3 cell lysate.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable at 2-8°C for 1 year from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,05-1076,Anti-c-Jun Antibody, clone 6E4.4

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