- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,04-1524,Anti-Cdt1 Antibody, clone PKS136, 1-104
产品名称：Anti-Cdt1 Antibody, clone PKS136, 1-104
产品型号：04-1524
Anti-Cdt1 Antibody, clone PKS136, 1-104 is a Mouse Monoclonal Antibody for detection of Cdt1 also known as DNA replication factor, Double parked homolog & has been validated in WB & IP.
merck millipore,默克密理博,04-1524,Anti-Cdt1 Antibody, clone PKS136, 1-104
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 04-1524 描述 Anti-Cdt1 Antibody, clone PKS136, 1-104 Alternate Names - chromatin licensing and DNA replication factor 1
- DNA replication factor
- Double parked homolog
- Double parked, Drosophila, homolog of
背景信息 Cdt1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. Cdt1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, Cdt1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. Cdt1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006). 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-Cdt1 Antibody, clone PKS136, 1-104 is a Mouse Monoclonal Antibody for detection of Cdt1 also known as DNA replication factor, Double parked homolog & has been validated in WB & IP. 主要应用 - Western Blotting
应用说明 Immunoprecipitation: A previous lot of this antibody was used in IP. 生物信息 免疫原品种 His-tagged recombinant human protein corresponding to human Cdt1. 克隆 PKS136, 1-104 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes Cdt1 同种型 IgG2bκ 品种反应性 Human Species Reactivity Note Reacts with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_112190.2
基因符号 - RIS2
- CDT1
- DUP
纯化方法 Protein G purfied UniProt编号 - Q9H211
UniProt汇总 FUNCTION: Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene.
SUBUNIT STRUCTURE: Interaction with GMNN inhibits both binding of the MCM complex to origins of replication and DNA-binding activity.
SUBCELLULAR LOCATION: Nucleus.
DEVELOPMENTAL STAGE: Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
PTM : Ubiquitinated, leading to its subsequent degradation. The interaction with GMNN protects it against ubiquitination.
Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.
SEQUENCE SIMILARITIES: Belongs to the Cdt1 family.
SEQUENCE CAUTION: The sequence AF070552 differs from that shown. Reason: Frameshift at positions 278 and 312.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected Cdt1 on 10 µg of HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,203642,BMP-4, Human, Recombinant, E. coli
下一件merck millipore产品：merck millipore,默克密理博,AB5084P,Anti-Dopamine D2 Receptor Antibody
相关产品
merck millipore,默克密理博,03-251,RIPAb+™ IGF2BP2 Antibody,03-251
merck millipore,默克密理博,ABD80,Anti-CRTAC1 Antibody
merck millipore,默克密理博,4500-0455,Guava Nexin Reagent for Flow Cytometry - 500 tests
merck millipore,默克密理博,4700-0050,Guava ViaCount Cell Dispersal Reagent for Flow Cytometry
merck millipore,默克密理博,203642,BMP-4, Human, Recombinant, E. coli
merck millipore,默克密理博,AB5084P,Anti-Dopamine D2 Receptor Antibody
merck millipore,默克密理博,MAB10210,Anti-Disialosyl LEA Antigen Antibody, clone FH7
merck millipore,默克密理博,GF435,HumanKine OSM, Human Recombinant Xeno-Free
merck millipore,默克密理博,04-397,Anti-PDGF Receptor Beta (CT) Antibody, clone Y92, rabbit monoclonal