merck millipore,默克密理博,03-230,SIRT5 (His-tag) human recombinant

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,03-230,SIRT5 (His-tag) human recombinant - HDAC protein
产品名称：SIRT5 (His-tag) human recombinant - HDAC protein
产品型号：03-230
Human recombinant SIRT5 (His-tag) expressed in E. Coli.

merck millipore,默克密理博,03-230,SIRT5 (His-tag) human recombinant - HDAC protein

产品介绍

merck millipore,默克密理博,03-230,SIRT5 (His-tag) human recombinant - HDAC protein

重要规格表

主要应用	品种
EA	Human

描述
产品目录编号	03-230
品牌系列	Upstate
商名	Upstate
描述	SIRT5 (His-tag) human recombinant - HDAC protein
概述	Human recombinant SIRT5 (His-tag) expressed in E. Coli
背景信息	SIRT5, along with SIRT 3 and 4, is one of three human mitochondrial sirtuins (homologs of yeast Sir2). SIRT5, which is localized to both the mitochondrial matrix and the intermembrane space, is an NAD+-dependent lysine deacetylase (class III HDAC). SIRT5’s only known physiological target is the intermembrane space protein, cytochrome c, suggesting the possibility of regulatory roles in metabolism and/or apoptosis. SIRT5 has also been shown to deacetylate and thereby upregulate the matrix urea cycle enzyme carbamoyl phosphate synthetase 1. This implies an important role for SIRT5 in ammonia detoxification and adaptation to elevated amino acid catabolism during starvation, calorie restriction, or a high protein diet. SIRT5 is most strongly expressed in thymus, lymphoblasts, and heart muscle cells and its chromosomal location (6p23) has been implicated in chromosomal abnormalities associated with malignancies.

产品信息
格式	Purified
演示	In liquid containing 25 mM Tris, pH 7.5, 100 mM NaCl, 5 mM DTT, containing 10% glycerol.

应用
应用	Human recombinant SIRT5 (His-tag) expressed in E. Coli.
主要应用	Enzyme Assay
应用说明	HDAC deacetylase assays

生物信息
宿主	E. coli
纯度	Purity >95% by SDS-PAGE
来源	Recombinant residues 37-310 of human SIRT5 isoform 1, with an N-terminal His-tag
品种	Human
特异活性	>200 pmol/min/mg at 37ºC, as determined by a fluorometric activity assay.
物理形式	Frozen solution

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the SIRT5 into separate tubes before storing at -80°C.

储存和货运信息

存储条件

Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the SIRT5 into separate tubes before storing at -80°C.