merck millipore,默克密理博,03-203,RIPAb+ Gemin6 - RIP Validated Antibody and Primer Set

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merck millipore,默克密理博,03-203,RIPAb+ Gemin6 - RIP Validated Antibody and Primer Set
产品名称：RIPAb+ Gemin6 - RIP Validated Antibody and Primer Set
产品型号：03-203
This RIPAb+ Gemin6 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.

merck millipore,默克密理博,03-203,RIPAb+ Gemin6 - RIP Validated Antibody and Primer Set

产品介绍

merck millipore,默克密理博,03-203,RIPAb+ Gemin6 - RIP Validated Antibody and Primer Set

重要规格表

品种反应性	主要应用
H	ICC, WB

描述
产品目录编号	03-203
品牌系列	Upstate
商名	Upstate RIPAb+
描述	RIPAb+ Gemin6 - RIP Validated Antibody and Primer Set
概述	RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation. Gemin6 is part of a large macromolecular complex that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN, Gemin 2, Gemin 3, Gemin 4, and Gemin 5.
Alternate Names	gemin 6 gem (nuclear organelle) associated protein 6
背景信息	The survival of motor neurons (SMN) complex is essential for the biogenesis of small nuclear ribonucleoprotein (snRNP) complexes in eukaryotic cells. Reduced levels of SMN cause the motor neuron degenerative disease, spinal muscular atrophy. Complexes containing Gemin3-Gemin4-Gemin5 and Gemin6-Gemin7-unrip persist at similar levels when SMN is reduced. Gemin5-containing subunits bind small nuclear RNA independently of the SMN complex and without a requirement for exogenous ATP. ATP hydrolysis is, however, required for displacement of small nuclear RNAs from the Gemin5-containing subunits and their assembly into snRNPs.

产品信息
格式	Purified
控制	Includes negative control normal mouse IgG antibody and control primers specific for the cDNA of human U1snRNA.
演示	Anti-Gemin6 (Mouse Monoclonal). One vial containing 50 μg of purified mouse IgG1k in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide and 30% glycerol. Store at -20°C. Normal Mouse IgG. One vial containing 125 µg of purified mouse IgG in 125 µL of storage buffer containing 0.1% sodium azide. Store at -20°C. RIP Primers, U1snRNA. One vial containing 75 μL of 5 μM of each primer specific for the cDNA of U1 snRNP. Store at -20°C. FOR: GGG AGA TAC CAT GAT CAC GAA GGT REV: CCA CAA ATT ATG CAG TCG AGT TTC CC

应用
应用	This RIPAb+ Gemin6 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunoprecipitation from RIP lysate: Representative lot data. RIP lysate from HeLa cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using either 5 µg of a normal mouse IgG or 5 µg of Anti-Gemin6 antibody. Ten percent of the precipitated proteins (lane 1: mouse IgG, lane 2: Gemin6) were resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Gemin6 antibody (1:5000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232) Arrow indicates Gemin6 (Please see figures). Western Blot Analysis: Representative lot data. HeLa cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-Gemin6 (0.1 µg/mL). Proteins were visualized using a Goat Anti-Mouse IgG conjugated to HRP and chemiluminescence detection system. Arrow indicates Gemin6 (~16 kDa) (Please see figures). Immunoprecipitation: A representative lot was used by an independent laboratory for IP (Battle, D.J., et al. (2007). The Journal of Biological Chemistry. 282:27953-27959).

生物信息
免疫原品种	Recombinant protein corresponding to human Gemin6.
宿主	Mouse
特异性	Recognizes Gemin6.
同种型	IgG1κ
品种反应性	Human
Species Reactivity Note	Demonstrated to react with human.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_079051.9
Entrez基因汇总	GEMIN6 is part of a large macromolecular complex, localized to both the cytoplasm and the nucleus, that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN (MIM 600354), GEMIN2 (SIP1; MIM 602595), GEMIN3 (DDX20; MIM 606168), GEMIN4 (MIM 606969), and GEMIN5 (MIM 607005).[supplied by OMIM].
基因符号	SIP2 GEMIN6
纯化方法	Protein G purfied
UniProt编号	Q8WXD5
UniProt汇总	FUNCTION: The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. SUBUNIT STRUCTURE: Part of the core SMN complex that contains SMN1, SIP1/GEMIN2, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts directly with GEMIN7, SNRPB, SNRPD2, SNRPD3 and SNRPE. SUBCELLULAR LOCATION: Nucleus › nucleoplasm. Nucleus › gem. Cytoplasm. Note: Found both in the nucleoplasm and in nuclear bodies called gems (Gemini of Cajal bodies) that are often in proximity to Cajal (coiled) bodies. Also found in the cytoplasm. SEQUENCE CAUTION: The sequence BAB15660.1 differs from that shown. Reason: Frameshift at position 142.

产品使用声明
质量保证	RNA Binding Protein Immunoprecipitation: RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using either 5 µg of a normal mouse IgG or 5 µg of Anti-Gemin6 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700). Successful immunoprecipitation of Gemin6-associated RNA was verified by qPCR using RIP Primers U1snRNA (Please see figures). Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

RNA Binding Protein Immunoprecipitation:
RIP Lysate prepared from HeLa cells (2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using either 5 µg of a normal mouse IgG or 5 µg of Anti-Gemin6 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of Gemin6-associated RNA was verified by qPCR using RIP Primers U1snRNA (Please see figures).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.