• 德国mn,macherey-nagel,740966.10,NucleoSpin TriPrep(10 preps)从同一样本中同时提取DNA,RNA和蛋白
  • 德国mn,macherey-nagel,740966.10,NucleoSpin TriPrep(10 preps)从同一样本中同时提取DNA,RNA和蛋白

    产品名称:NucleoSpin TriPrep (10 preps)从同一样本中同时提取DNA,RNA和蛋白
    产品型号:740966.10
    MN 740966.10 NucleoSpin TriPrep (10 preps)单个柱子分离即用型RNA 、DNA和蛋白质,不受蛋白片段、定位及修饰的影响

    德国mn,macherey-nagel,740966.10,NucleoSpin TriPrep(10 preps)从同一样本中同时提取DNA,RNA和蛋白

  • 产品介绍
  • 德国mn,macherey-nagel,740966.10,NucleoSpin TriPrep (10 preps)从同一样本中同时提取DNA,RNA和蛋白

    Features
    Parallel isolation of RNA, DNA, and protein from undivided samples
    德国mn,macherey-nagel• Convenient one-column preparation of RNA, DNA, and protein
    • High quality RNA and DNA, ready to use for downstream applications
    • High protein yield independent of protein size, localization, modification, etc.
    • Easy protein quantification using the MACHEREY-NAGEL Protein Quantification Assay
    • Complete kit, including: NucleoSpin® Filters (shredders) for optimal lysis, 
       rDNase for on-column DNA digestion, Protein Solving Buffer for solving all types of proteins
    TechnologySilica-membrane technology
    FormatMini spin columns
    Sample material

    < 5 x 106 cells, <30 mg human/ animal Tissue, <100 mg plant


    RNADNAProtein
    Fragment size> 200 nt< 30 kbp15–300 kbp
    Typical yield< 70 µg< 6 µg< 1200 µg
    A260/A280

    1.9–2.1

    1.7–1.9–
    Typical RIN (RNA integrity number)> 9––
    Elution volume (RNA and DNA)
    Resolubilization volume (protein)
    40–120 µL
    100 µL10–100 µL
    Preparation time30 min/6 preps35 min/6 preps (RNA+DNA)30 min/6 preps
    Binding capacity200 µg10 µg*–

    Applications**

    • Rapid purification of total RNA, DNA, and protein from small and precious samples – no sample splitting for
       different isolations necessary
    • Gene expression profiling, analysis of transgenic organisms, drug screening, genotyping
    • Reliable interpretation of RNA, DNA, and protein amounts
    • Good preservation of protein primary structure and posttranslational modifications (e.g., protein
       phosphorylation) allows protein analysis without additional inhibitors (e.g., proteinase or phosphatase 
       inhibitors)
    • Suitable for a broad spectrum of starting materials, including cultured cells, tissue, bacteria, yeast, and 
       plants
    • RNA and DNA are ready to use for all typical downstream applications
    • Proteins are ready to use for SDS-PAGE / Western blotting
    * Binding capacity of DNA < 10 μg, strongly depending on RNA amount bound to the membrane.
    ** Kits to be used for research purposes only. DISTRIBUTION AND USE IN THE USA IS PROHIBITED FOR PATENT REASONS.


    德国mn,macherey-nagel,740966.10,NucleoSpin TriPrep (10 preps)从同一样本中同时提取DNA,RNA和蛋白

上一件macherey-nagel产品:德国mn,macherey-nagel,740966.50,NucleoSpin TriPrep(50 preps)从同一样本中同时提取DNA,RNA和蛋白
下一件macherey-nagel产品:德国mn,macherey-nagel,745510.1,Protino GST/4B Columns 1 ml(1)FPLC柱子,用于纯化GST标签蛋白



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