MN,Macherey-Nagel
  • 德国mn,macherey-nagel,740420.2,NucleoBond Xtra Midi EF Sample 质粒DNA纯化试剂盒

  • 德国mn,macherey-nagel,740420.2,NucleoBond Xtra Midi EF Sample 质粒DNA纯化试剂盒

    产品名称：NucleoBond Xtra Midi EF Sample 质粒DNA纯化试剂盒
    产品型号：740420.2


    德国mn,macherey-nagel,740420.2,NucleoBond Xtra Midi EF Sample 质粒DNA纯化试剂盒

  • 产品介绍
  • 德国mn,macherey-nagel,740420.2,NucleoBond Xtra Midi EF Sample 质粒DNA纯化试剂盒

    NucleoBond® Xtra Midi EF / Maxi EF

    特点：

    节约68 % 时间,增加100 % 的产量

    新一代阴离子交换技术制备超快，高产量，无内毒素的质粒DNA

    质粒DNA 中内毒素少于0.05 EU/μg DNA，适合对内毒素高敏感的细胞转染

    专利技术附加的洗涤步骤以去除内毒素-无需额外的冰上放置

    优化设计的柱子：制备时间约为30 分钟(Midiprep)；35 分钟(Maxi prep)

    包含柱过滤器：高过虑流速，清除裂解液中的不溶物的同时可向柱子加样

    改进的硅材料：标准的产量约为250 μg (Midi和) 1 000 μg (Max i)

    NucleoBond®Xtra Plus EF kits 包含NucleoBond®Finalizers可加速 DNA 沉淀

    产品概览：

    技术          阴离子交换色谱法

    产品形式      Midigravity-flow columns            Maxigravity-flow columns

    裂解液澄清    Columnfilters                       Columnfilters

    样品材料      <200 mL (high copy)                 <600 mL (high copy)

     <400 mL (low copy)                  < 1200 mL (low copy)

    载体大小      <300 kbp                            < 300 kbp

    产量           250 μ g                            1000μ g

    A260/A280   1.80 – 1.95                          1.80– 1.95

    内毒素水平   < 0.05 EU/μg DNA                    <0.05 EU/μg DNA

    异丙醇     Xtra Midi EF /Xtra Midi Plus EF    Xtra Maxi EF/Xtra Maxi Plus EF

    沉淀        Centrifugation                         NucleoBond®Finalizer

    制备时间    100min / 4 preps 45 min / 4 preps     105 min /4 preps 50 min / 4 preps

    应用：

    从大肠杆菌中提取高拷贝/低拷贝质粒

    订购信息

    产品

    规格

    备注

    货号

    NucleoBond® Xtra Midi EF kits

    NucleoBond® Xtra Midi EF

    10

    NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra

    Column Filters, buffers, RNase A

    740420.10

    50

    同上

    740420.50

    NucleoBond® XtraMidi Plus EF

    10

    NucleoBond® Xtra Midi Columns with inserted

    NucleoBond® Xtra Column Filters, NucleoBond®Finalizer,buffers,

    RNase A

    740422.10

    50

    同上

    740422.50

    NucleoBond® Xtra Maxi EF kits

    NucleoBond® Xtra Maxi EF

    10

    NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra

    Column Filters, buffers, RNase A

    740424.10

    50

    同上

    740424.50

    NucleoBond® Xtra Maxi Plus EF

    10

    NucleoBond® Xtra Maxi Columns with inserted

    NucleoBond® Xtra Column Filters, NucleoBond®Finalizer Large,

    buffers, RNase A

    740426.10

    50

    同上

    740426.50

    产品配件

    包装

    备注

    货号

    NucleoBond®Xtra Combi Rack

    for use with NucleoBond®Xtra Midi, Maxi,

    AX 100, 500, 2 000, and 10 000 Columns

    740415

    NucleoBond®Xtra EF Buffer Set I

    for isolation of endotoxin-free low-copy plasmids, cosmids,BACs,

    PACs, and P1 constructs,only applicable with NucleoBond® Xtra EF

    kits,sufficient for 10 Xtra EF Maxi preps or 20 Xtra EF Midi preps,

    set includes buffers RES-EF, LYS-EF, NEU-EF, and RNase A

    740427


    德国mn,macherey-nagel,740420.2,NucleoBond Xtra Midi EF Sample 质粒DNA纯化试剂盒

上一件macherey-nagel产品：德国mn,macherey-nagel,740771.20,NucleoSpin Plant II Midi(20) 植物基因组DNA提取试剂盒
下一件macherey-nagel产品：德国mn,macherey-nagel,740398.12,Exosome Precipitation Solution(Plasma)


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