merck millipore,默克密理博,MABT145,Anti-Calreticulin Antibody, clone EPR3924, rabbit monoclonal

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,MABT145,Anti-Calreticulin Antibody, clone EPR3924, rabbit monoclonal
产品名称：Anti-Calreticulin Antibody, clone EPR3924, rabbit monoclonal
产品型号：MABT145
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Calreticulin Antibody, clone EPR3924, Rabb More>>

merck millipore,默克密理博,MABT145,Anti-Calreticulin Antibody, clone EPR3924, rabbit monoclonal

产品介绍

merck millipore,默克密理博,MABT145,Anti-Calreticulin Antibody, clone EPR3924, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	FC, WB, ICC	Rb	Diluted Culture Supernatant	Monoclonal Antibody

描述
产品目录编号	MABT145
描述	Anti-Calreticulin Antibody, clone EPR3924, rabbit monoclonal
Alternate Names	Calregulin calreticulin Endoplasmic reticulum resident protein 60 Sicca syndrome antigen A (autoantigen Ro calreticulin) calregulin autoantigen Ro
背景信息	Calreticulin is a molecular Ca+ binding chaperone promoting folding, oligomeric assembly, and quality control in ER. Calreticulin is a highly versatile lectin-like chaperone, and it participates during the synthesis of a variety of molecules, including ion channels, surface receptors, integrins and transporters (1). Calreticulin indeed provides up to 45% of the Ca2+-buffering capacity for a pool of the IP3-sensitive Ca2+ inside the ER (2). Calreticulin also functions in cell adhesion, integrin-dependent Ca2+ signaling and steroid-sensitive gene expression (1).

产品信息
格式	Diluted Culture Supernatant
控制	SH-SY5Y, HL-60, HepG2, HeLa, fetal kidney, and fetal brain lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Calreticulin Antibody, clone EPR3924, Rabbit is validated for use in WB, IC, IH(P), FC, IP for the detection of Calreticulin.
主要应用	Flow Cytometry Western Blotting Immunocytochemistry
应用说明	Immunoprecipitation Analysis: 1:10 dilution from a representative lot was used in IP. Flow Cytometry Analysis: 1:10 dilution from a representative lot was used in FC. Immunohistochemistry Analysis: 1:250-1:500 dilution from a representative lot detected Calreticulin in human kidney tissue. Immunocytochemistry Analysis: 1:100-250 dilution from a representative lot was used to detect Calreticulin in HeLa cells.

生物信息
免疫原品种	Synthetic peptide corresponding to human Calreticulin.
克隆	EPR3924
宿主	Rabbit
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Demonstrated to react with human, mouse, and rat.
抗体类型	Monoclonal Antibody
基因符号	CRTC SSA FLJ26680 cC1qR RO CRT grp60 ERp60 HACBP CRP55
纯化方法	Unpurified
UniProt编号	P27797
UniProt汇总	FUNCTION: Molecular calcium-binding chaperone promoting folding, oligomeric assembly and quality control in the ER via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export. SUBUNIT STRUCTURE: Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 By similarity. Interacts with NR3C1 and TRIM21. SUBCELLULAR LOCATION: Endoplasmic reticulum lumen. Cytoplasm › cytosol. Secreted › extracellular space › extracellular matrix. Cell surface. Note: Also found in cell surface (T cells), cytosol and extracellular matrix. Associated with the lytic granules in the cytolytic T-lymphocytes. DOMAIN: Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity. The interaction with glycans occurs through a binding site in the globular lectin domain. The zinc binding sites are localized to the N-domain. Associates with PDIA3 through the tip of the extended arm formed by the P-domain. SEQUENCE SIMILARITIES: Belongs to the calreticulin family. CAUTION: Was originally (Ref.1) thought to be the 52 kDa Ro autoantigen. MASS SPECTROMETRY: Molecular mass is 46879 Da from positions 18 - 417. Determined by MALDI.

产品使用声明
质量保证	Evaluated by Western Blot in SH-SY5Y, HL-60, HepG2, HeLa, fetal kidney, and fetal brain lysates Western Blot Analysis: 1:1,000-10,000 dilution of this antibody detected Calreticulin in SH-SY5Y, HL-60, HepG2, HeLa, fetal kidney, and fetal brain lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing Solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below
-20°C may cause glycerol containing Solutions to become frozen during storage.