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merck millipore,默克密理博,MAB5290,Anti-Dopamine D1A Receptor Antibody, clone SG2-D1a
产品名称：Anti-Dopamine D1A Receptor Antibody, clone SG2-D1a
产品型号：MAB5290
Anti-Dopamine D1A Receptor Antibody, clone SG2-D1a detects level of Dopamine D1A Receptor & has been published & validated for use in IH & WB.
merck millipore,默克密理博,MAB5290,Anti-Dopamine D1A Receptor Antibody, clone SG2-D1a
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Sh, M, R WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB5290 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Dopamine D1A Receptor Antibody, clone SG2-D1a 产品信息 格式 Purified 演示 Purified immunoglobulin. Liquid in 0.02 M Phosphate buffer, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide. 应用 应用 Anti-Dopamine D1A Receptor Antibody, clone SG2-D1a detects level of Dopamine D1A Receptor & has been published & validated for use in IH & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Western blot: 1:250-1:500 on unboiled membrane fractions from rat caudate tissue. Reactivity is also seen in perfrontal and temporal cortex. An identified band between 45-66kDa is observed (Luedtke, 1999).
Immunohistochemistry: 4% PFA fixed, vibratome sections, blocked with 3% NGS, 1% BSA with 0.2% triton X-100. Primary antibodies diluted onlyin PBS with excess blocking reagents.
Optimal working dilutions must be determined by the end user.
SUGGESTED WESTERN BLOT PROTOCOL
Solutions
10X Transfer buffer 1 liter
Tris 115.3 gm
Glycine 24.2 gm
[SDS 4 gm (may be omitted)]
For a 1X working solution: 1 liter
mix 700 mL water
200 mL MeOH
100 mL 10X TransferTBST
stock 1 liter
1M Tris pH 7.5 10 mL
5 M NaC 30 mL
10% Tween-20 5 mL
1. Run SDS-PAGE gel as desired.
2. Soak PVDF membrane (ImmobilonP from Millipore) in 100% methanol for 1-2 minutes to wet the membrane.
3. Soak membrane in transfer buffer (see below) until ready to set up blot transfer (>15 min).
4. Assemble gel-transfer sandwich with the blot toward the positive electrode.
5. Transfer protein from gel to membrane at 100 volts for 1-2 hours for small molecular weight proteins or 65 mA for 16 hours (overnight) for complete transfer of higher molecular weight proteins.
6. Stain the transferred bands with Chemicon BLOT-FastStain™ (Catalog Number 2076).
7. Destain with deionized water.
8. Block with 5% non-fat milk (Marvel or Carnation) in TBS or PBS, overnight at 4°C. The non-fat milk should be dissolved freshly, centrifuged 10,000 rpm for 10 min, and filtered through glass filter (Gelman Acrodisc).
9. Incubation with first antibody overnight with gentle agitation at 4°C.
Optimal working dilutions and incubation time will need to be determined by the end user.
10. Wash at least 3 x 5 min. with TBST. From this stage, azide should be omitted.
11. Incubation with the secondary antibody (HRP-conjugated goat anti-mouse antibody, for example Chemicon Catalog Number AP124P, diluted appropriately) 1 h at room temperature.
12. Wash at least 3 x 5 min. with TBST.
13. Perform ECL with commercial kit (femtoLUCENT, Chemicon Catalog Number 2078).生物信息 免疫原品种 Recombinant rat D1a Receptor, carboxy terminus. 克隆 SG2-D1a 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Dopamine Receptor D1A, no cross reactivity to D1B (D5) receptor. Immunoreactivity was found to be specific for mouse and rat D1A receptor. Human, pig, dog, rabbit, sheep and bovine samples were non-reactive by western analysis. 同种型 IgG2b 品种反应性 SheepMouseRat 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000794.3
Entrez基因汇总 This gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene. 基因符号 - D
- DRD1
- DRD1A
- DADR
非反应性品种 - Rabbit
- Bovine
- Canine
- Human
- Pig
UniProt编号 - P21728
UniProt汇总 FUNCTION: SwissProt: P21728 # This is one of the five types (D1 to D5) of receptors for dopamine. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
SIZE: 446 amino acids; 49293 Da
SUBUNIT: Interacts with DNAJC14 via its C-terminus (By similarity). Interacts with DRD1IP.
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity). Note=Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14 (By similarity).
TISSUE SPECIFICITY: Detected in caudate, nucleus accumbens and in the olfactory tubercle.
SIMILARITY: SwissProt: P21728 ## Belongs to the G-protein coupled receptor 1 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,113200,Anti-α-Actin Mouse mAb (1A4)
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