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merck millipore,默克密理博,HTS081M,ChemiScreen™ Membrane Preparation Recombinant Human TBXA2R (TP) Thromboxane Receptor
产品名称：ChemiScreen™ Membrane Preparation Recombinant Human TBXA2R (TP) Thromboxane Receptor
产品型号：HTS081M
Human TP / TXA2 / PGH2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS081M,ChemiScreen™ Membrane Preparation Recombinant Human TBXA2R (TP) Thromboxane Receptor
- 产品介绍
- merck millipore,默克密理博,HTS081M,ChemiScreen™ Membrane Preparation Recombinant Human TBXA2R (TP) Thromboxane Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay, and GTPγS binding. Human Chem-1 TP / TXA2 / PGH2 描述 产品目录编号 HTS081M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiScreen™ Membrane Preparation
Recombinant Human TBXA2R (TP) Thromboxane Receptor概述 Human Full-length human TBXA2R cDNA encoding the TP short form (TPα) 背景信息 TP receptors (Thromboxane A2 receptors) are widely distributed among different organ systems and have been localized on both cell membranes and intracellular structures. TP receptors belong to G protein-coupled receptor family (Hirata et al., 1991). Activation of TP receptors induces platelet aggregation, vascular and respiratory smooth muscle constriction, and enhances mitogenic responses of vascular smooth muscle cells that are stimulated by growth factors (Ali et al, 1993; Hanasaki et al., 1990). The human TP receptor is encoded by a single gene that is alternatively spliced at the carboxyl terminus, resulting in two isoforms, TPα (343 residues) and TPβ (407 residues). Both isoforms couple to Gq pathway, but couple oppositely to adenylate cyclase. The cDNA encoding human TPα has been stablely expressed in the Chem-1 host, which supports high levels of recombinant receptor expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. The membrane preparations exhibit a Kd of 9.19 nM for [3H]-SQ 29548. With 10 nM [3H]-SQ 29548, 10 ug/well TP Membrane Prep yields greater than 5-fold signal-to-background ratio. 产品信息 演示 One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 5-fold signal:background with 3H-labeled SQ 29548 at 15 nM. Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives. Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C. 应用 应用 Human TP / TXA2 / PGH2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay, and GTPγS binding. 生物信息 品种 Human 品种反应性 Human Only Entrez基因编号 - NM_201636.1
- NM_001060.4
基因符号 - TXA2-R
- TBXA2R
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 TP / TXA2 / PGH2 靶子系列 Prostanoid UniProt编号 - P21731
UniProt汇总 FUNCTION: SwissProt: P21731 # Receptor for thromboxane A2 (TXA2), a potent stimulator of platelet aggregation. The activity of this receptor is mediated by a G-protein that activate a phosphatidylinositol-calcium second messenger system. In the kidney, the binding of TXA2 to glomerular TP receptors causes intense vasoconstriction.
SIZE: 369 amino acids; 40092 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
DISEASE:SwissProt: P21731 # Defects in TBXA2R are the cause of a dominantly inherited bleeding disorder [MIM:188070].
SIMILARITY: SwissProt: P21731 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50 mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [3H]-SQ 29548 (Perkin Elmer # NET936)
Wash Buffer: 50 mM Hepes, pH 7.4, 500 mM NaCl , 0.1% BSA, filtered and stored at 4°C.产品使用声明 质量保证 Signal:background and specific binding values obtained in a competition binding assay with varying amounts of Y2 membrane prep:
10 µg/well 5 µg/well Signal:Background 6.6 4.6 Specific Binding (cpm) 2090 963
1 unit = 10 μg membrane preparation
Bmax: 13.9 pmol/mg
Kd: 14.6 nM
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw. 包装信息 数量 2 mL 包装 200 units
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