merck millipore,默克密理博,04-1129,Anti-Casein Kinase II (alpha subunit) Antibody, clone EP1963Y, rabbit monoclonal

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merck millipore,默克密理博,04-1129,Anti-Casein Kinase II (alpha subunit) Antibody, clone EP1963Y, rabbit monoclonal
产品名称：Anti-Casein Kinase II (alpha subunit) Antibody, clone EP1963Y, rabbit monoclonal
产品型号：04-1129
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-1129,Anti-Casein Kinase II (alpha subunit) Antibody, clone EP1963Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1129,Anti-Casein Kinase II (alpha subunit) Antibody, clone EP1963Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	FC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1129
描述	Anti-Casein Kinase II (alpha subunit) Antibody, clone EP1963Y, rabbit monoclonal
Alternate Names	protein kinase CK2 CK II CK2 catalytic subunit alpha casein kinase 2, alpha 1 polypeptide casein kinase II alpha 1 subunit
背景信息	Unlike most serine/threonine kinases, Casein Kinases (CKs) are not activated by specific stimuli, but are essentially constitutively active. CK1 autophosphorylates, causing inactivation, and protein phosphatases reactivate the kinase. The substrates of CKs are numerous, but their function is less clear, perhaps due to the absence of obvious regulation. CK1 isoforms are thought to be involved in the response to DNA damage, while CK2 levels are elevated in many types of cancer.

产品信息
格式	Unpurified
控制	HeLa cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl containing 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Flow Cytometry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected Casein Kinase II (alpha subunit) in human breast carcinoma tissue. Flow Cytometry: A 1:20 dilution of a previous lot was used in flow cytometry.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the N-terminus of human Casein Kinase II (alpha subunit).
表位	N-terminus
克隆	EP1963Y
宿主	Rabbit
特异性	This antibody recognizes Casein Kinase II (alpha subunit).
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_001895.3
基因符号	OTTHUMP00000029926 CK2A1 CKII EC 2.7.11.1 OTTHUMP00000029925
纯化方法	Unpurified
UniProt编号	P68400
UniProt汇总	FUCNTION: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. The alpha and alpha' chains contain the catalytic site. Participates in Wnt signaling. CK2 phosphorylates 'Ser-392' of p53/TP53 following UV irradiation. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. SUBUNIT STRUCTURE: Tetramer composed of an alpha chain, an alpha' and two beta chains. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with RNPS1. SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CK2 subfamily. Contains 1 protein kinase domain.

产品使用声明
质量保证	Evaluated by Western Blot on HeLa cell lysates. Western Blot Analysis: A 1:500-1,000 dilution of this antibody was used to detect Casein Kinase II (alpha subunit) in HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.