德国mn,macherey-nagel,740424.2,NucleoBond Xtra Maxi EF Sample(2) 质粒DNA纯化试剂盒

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MN Macherey-Nagel

德国mn,macherey-nagel,740424.2,NucleoBond Xtra Maxi EF Sample (2) 质粒DNA纯化试剂盒

NucleoBond® Xtra Midi EF / Maxi EF

特点：

节约68 % 时间,增加100 % 的产量

新一代阴离子交换技术制备超快，高产量，无内毒素的质粒DNA

质粒DNA 中内毒素少于0.05 EU/μg DNA，适合对内毒素高敏感的细胞转染

专利技术附加的洗涤步骤以去除内毒素-无需额外的冰上放置

优化设计的柱子：制备时间约为30 分钟(Midiprep)；35 分钟(Maxi prep)

包含柱过滤器：高过虑流速，清除裂解液中的不溶物的同时可向柱子加样

改进的硅材料：标准的产量约为250 μg (Midi和) 1 000 μg (Max i)

NucleoBond®Xtra Plus EF kits 包含NucleoBond®Finalizers可加速 DNA 沉淀

产品概览：

技术阴离子交换色谱法

产品形式 Midigravity-flow columns Maxigravity-flow columns

裂解液澄清 Columnfilters Columnfilters

样品材料 <200 mL (high copy) < 600 mL (high copy)

<400 mL (low copy) < 1200 mL (low copy)

载体大小 <300 kbp < 300 kbp

产量 250 μ g 1000μg

A260/A280 1.80 – 1.95 1.80– 1.95

内毒素水平 < 0.05 EU/μg DNA <0.05 EU/μg DNA

异丙醇 Xtra Midi EF/Xtra Midi Plus EF Xtra Maxi EF/Xtra Maxi Plus EF

沉淀 Centrifugation NucleoBond®Finalizer

制备时间 100min / 4 preps 45 min / 4 preps 105 min /4 preps 50 min / 4 preps

应用：

从大肠杆菌中提取高拷贝/低拷贝质粒

订购信息

产品	规格	备注	货号
NucleoBond® Xtra Midi EF kits
NucleoBond® Xtra Midi EF	10	NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A	740420.10
	50	同上	740420.50
NucleoBond® XtraMidi Plus EF	10	NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer,buffers, RNase A	740422.10
	50	同上	740422.50
NucleoBond® Xtra Maxi EF kits
NucleoBond® Xtra Maxi EF	10	NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, buffers, RNase A	740424.10
	50	同上	740424.50
NucleoBond® Xtra Maxi Plus EF	10	NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra Column Filters, NucleoBond® Finalizer Large, buffers, RNase A	740426.10
	50	同上	740426.50
产品配件	包装	备注	货号
NucleoBond®Xtra Combi Rack		for use with NucleoBond® Xtra Midi, Maxi, AX 100, 500, 2 000, and 10 000 Columns	740415
NucleoBond®Xtra EF Buffer Set I		for isolation of endotoxin-free low-copy plasmids, cosmids,BACs, PACs, and P1 constructs,only applicable with NucleoBond® Xtra EF kits,sufficient for 10 Xtra EF Maxi preps or 20 Xtra EF Midi preps, set includes buffers RES-EF, LYS-EF, NEU-EF, and RNase A	740427