merck millipore,默克密理博,SCR082,MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit

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merck millipore,默克密理博,SCR082,MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit
产品名称：MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit
产品型号：SCR082
MilliTrace Constitutive GFP Reporter Mouse ES Cell Kit provides ready-to-use mouse embryonic stem cells that are constitutively labeled with the hmGFP along with expansion medium to help maintain expr More>>

merck millipore,默克密理博,SCR082,MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit

产品介绍

merck millipore,默克密理博,SCR082,MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit

描述
产品目录编号	SCR082
商名	Chemicon MilliTrace
描述	MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit
概述	MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit provides ready-to-use mouse embryonic stem cells that are constitutively labeled with the humanized mulleri green fluorescent protein (hmGFP) along with expansion medium to help maintain expression of the transgene. MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cells were generated by transfection of C57/BL6 mouse embryonic stem cells with a proprietary bicistronic plasmid construct containing hmGFP under the control of a constitutive chicken actin promoter. FACS analyses of stable transfectants indicate that greater than 95% of the cells express GFP at high levels even after 10 passages. MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cells display the immunochemical staining properties of pluripotent stem cells and are thus positive for alkaline phosphatase, Oct-4, Sox-2, and SSEA-1. Under neuronal differentiation conditions, cells can be differentiated to βIII-tubulin positive neurons (GFP+βIII-tubulin+) (Use Mouse Embryonic Stem Cell Neurogenesis Kit, Catalog No. SCR101).

产品信息
部件	MilliTrace Mouse Embryonic Stem Cell Expansion Medium (Catalog No. SCM042) MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cells, 1 x 10⁶ viable cells

应用
应用	MilliTrace Constitutive GFP Reporter Mouse ES Cell Kit provides ready-to-use mouse embryonic stem cells that are constitutively labeled with the hmGFP along with expansion medium to help maintain expression of the transgene.

生物信息
细胞系类型	Embryonic Stem Cells

产品使用声明
质量保证	Constitutive GFP reporter mouse embryonic stem cells have been validated for high level constitutive expression of GFP, for high level of expression of alkaline phosphatase, Oct-4, Sox-2, and SSEA-1, and for their self-renewal and multi-lineage differentiation capacities. Cells display normal karyotype as assessed by G-banding of twenty metaphase cells and tested negative for mycoplasma.
使用声明	For Research Use Only. EMD Millipore does not recommend that a user derive clones from cells provided with this kit. However, should a user decide to do so, they may need a license to one or more third party patents and it is incumbent upon that user to determine whether and if so which third party patents need to be licensed. This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

产品使用声明

质量保证

Constitutive GFP reporter mouse embryonic stem cells have been validated for high level constitutive expression of GFP, for high level of expression of alkaline phosphatase, Oct-4, Sox-2, and SSEA-1, and for their self-renewal and multi-lineage differentiation capacities. Cells display normal karyotype as assessed by G-banding of twenty metaphase cells and tested negative for mycoplasma.

使用声明

For Research Use Only. EMD Millipore does not recommend that a user derive clones from cells provided with this kit. However, should a user decide to do so, they may need a license to one or more third party patents and it is incumbent upon that user to determine whether and if so which third party patents need to be licensed.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck-Millipore to request certain information about you and the establishment where the GMOs are being handled.

储存和货运信息
存储条件	· MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cells (Part No. SCC082) should be stored in Liquid Nitrogen. We recommend that the cells be used within ten passages. · EmbryoMax Complete ES Cell Medium with 15% FBS and LIF (Part No. ES-101-B) should be stored at -20°C until expiration date on the label. Upon thawing the medium should be stored at 2-8°C and given a 1-month expiration dating. · 5 mg/mL Puromycin Solution (Part No. CS201361) should be stored in working aliquots at -20°C for up to 2 years.

储存和货运信息

存储条件

· MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cells (Part No. SCC082) should be stored in Liquid Nitrogen. We recommend that the cells be used within ten passages.
· EmbryoMax Complete ES Cell Medium with 15% FBS and LIF (Part No. ES-101-B) should be stored at -20°C until expiration date on the label. Upon thawing the medium should be stored at 2-8°C and given a 1-month expiration dating.
· 5 mg/mL Puromycin Solution (Part No. CS201361) should be stored in working aliquots at -20°C for up to 2 years.

包装信息
数量	1 kit

merck millipore,默克密理博,SCR082,MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit

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merck millipore,默克密理博,SCR082,MilliTrace Constitutive GFP Reporter Mouse Embryonic Stem Cell Kit

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