merck millipore,默克密理博,QIA39,FragEL™ DNA Fragmentation Detection Kit, Fluorescent

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merck millipore,默克密理博,QIA39,FragEL™ DNA Fragmentation Detection Kit, Fluorescent - TdT Enzyme
产品名称：FragEL™ DNA Fragmentation Detection Kit, Fluorescent - TdT Enzyme
产品型号：QIA39

merck millipore,默克密理博,QIA39,FragEL™ DNA Fragmentation Detection Kit, Fluorescent - TdT Enzyme

产品介绍

merck millipore,默克密理博,QIA39,FragEL™ DNA Fragmentation Detection Kit, Fluorescent - TdT Enzyme

重要规格表

检测方法
Fluorescence

描述
概述	Allows for detection of apoptosis at the individual cell level. Apoptosis morphology is easily detected. Detection is by flow cytometry or fluorescence microscopy. The kit mounting media permits the observance of the total cell population.
概述	Cat. No. QIA39 is a fluorescein-conjugated version of our widely referenced TdT Colorimetric FragEL™ DNA Fragmentation Detection Kit (Cat. No. QIA33) for non-isotopic labeling. Terminal deoxynucleotidyl transferase (TdT) binds to exposed 3′-OH ends of DNA fragments generated in response to apoptotic signals and catalyzes the addition of fluorescein-labeled and unlabeled deoxynucleotides. When excited, fluorescein generates an intense signal which can be detected by fluorescence microscopy or flow cytometry. The mounting media sustains the fluorescent signal from samples labeled on slides and, therefore, aids in the morphological evaluation and characterization of normal and apoptotic cells. Non-apoptotic cells can be visualized by a DAPI filter.
产品目录编号	QIA39
品牌系列	Calbiochem®
同义词	TUNEL Assay

参考
参考	Darzynkiewicz, Z., et al. 1997. Cytometry 27, 1. Frey, T. 1997. Cytometry 28, 253. Shapiro, H.M. 1995. Practical Flow Cytometry, Third Edition. Wiley-Liss Inc., New York, New York. Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795. Gavrieli, Y., et al. 1992. J. Cell Biol. 119, 493. Fawthrop, D. J., et al. 1991. Arch. Toxicol. 65, 437. Martin, S.J., et al. 1990. J. Immunology 145, 1859. Wyllie, A. H. 1980. Nature 284, 555. Kerr, J. F. R., et al. 1972. Br. J. Cancer 26, 239.

参考

Darzynkiewicz, Z., et al. 1997. Cytometry 27, 1.
Frey, T. 1997. Cytometry 28, 253.
Shapiro, H.M. 1995. Practical Flow Cytometry, Third Edition. Wiley-Liss Inc., New York, New York.
Darzynkiewicz, Z., et al. 1992. Cytometry 13, 795.
Gavrieli, Y., et al. 1992. J. Cell Biol. 119, 493.
Fawthrop, D. J., et al. 1991. Arch. Toxicol. 65, 437.
Martin, S.J., et al. 1990. J. Immunology 145, 1859.
Wyllie, A. H. 1980. Nature 284, 555.
Kerr, J. F. R., et al. 1972. Br. J. Cancer 26, 239.

产品信息
检测方法	Fluorescence
形式	50 Tests
格式	Flow cytometry or fluorescence microscopy
试剂盒包含	Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Control Slides, Mounting Media (Cat. No. HC08), and a user protocol.

应用
主要应用	Flow Cytometry

生物信息
化验时间	2 h
样品类型	Frozen or paraffin sections, fixed cell preparations or cell suspensions
品种	a broad range of species

安全信息
R相	R: 23/25-34-36/37/38-42/43-45-61 Toxic by inhalation and if swallowed. Causes burns. Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation and skin contact. May cause cancer. May cause harm to the unborn child.
S相	S: 26-27-36/37/39-45-53 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Take off immediately all contaminated clothing. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). Avoid exposure - obtain special instructions before use.

产品使用声明
预定用途	The Calbiochem^® Fluorescein-FragEL™ DNA Fragmentation Detection Kit is a non-isotopic system for the labeling of DNA breaks in apoptotic nuclei in paraffin-embedded tissue sections, tissue cryosections, in cell preparations fixed on slides, or in cell suspensions. Detection and analysis of labeling is possible by flow cytometry or fluorescence microscopy.

储存和货运信息
货运代码	Dry Ice Only
毒性	Multiple Toxicity Values, refer to MSDS
有害材料注意事项：	Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存	-20°C
存储条件	Fluorescein-FragEL™ kit components are shipped on dry ice. Upon receipt, store kit at -20°C in a non-frost-free freezer. To avoid reagent loss in tube caps, briefly pulse spin all thawed solutions before removing caps. (see General Considerations below).
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No

补充信息
试剂盒包含	Proteinase K, Equilibration and Labeling Buffers, TdT Enzyme (Cat. No. PF060), Control Slides, Mounting Media (Cat. No. HC08), and a user protocol.