merck millipore,默克密理博,MABN699,Anti-Metadherin Antibody, clone 2F11C3

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merck millipore,默克密理博,MABN699,Anti-Metadherin Antibody, clone 2F11C3
产品名称：Anti-Metadherin Antibody, clone 2F11C3
产品型号：MABN699
Detect Metadherin using this mouse monoclonal antibody, Anti-Metadherin Antibody, clone 2F11C3 validated for use in western blotting, IHC & Flow Cytometry.

merck millipore,默克密理博,MABN699,Anti-Metadherin Antibody, clone 2F11C3

产品介绍

merck millipore,默克密理博,MABN699,Anti-Metadherin Antibody, clone 2F11C3

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	FC, WB, IHC	M	Affinity Purified	Monoclonal Antibody

描述
产品目录编号	MABN699
描述	Anti-Metadherin Antibody, clone 2F11C3
Alternate Names	Metastasis adhesion protein Protein LYRIC 3D3/LYRIC Astrocyte elevated gene-1 protein AEG-1 Lysine-rich CEACAM1 co-isolated protein Metadherin
背景信息	Metadherin, or more commonly known as LYRIC, or AEG-1 (Astrocyte elevated gene-1) is a single pass membrane protein that plays a key role in anchorage-independent growth of immortalized melanocytes and astrocytes. Anchorage-independent division is a critical component in tumor cell expansion and metastasis. Similarly, metadherin activates the nuclear factor kappa- B (NF-kappa-B) transcription leading to expression of numerous genes involved in cell growth and expansion, and it is also involved in HIF-1alpha mediated angiogenesis in tumor growth. Finally new research demonstrates that metadherin interacts with RNA-induced silencing complex (RISC) and plays a very important role in RISC and miRNA functions as well. The protein is a key target of research because of its tumor promoting effects in lung, bone and brain cancers. Metadherin is localized to the endoplasmic reticulum and nuclear membranes in normal tissue, thus staining is perinuclear primarily but Metadherin staining can also be seen in the nucleus, at tight junctions and even at the cell surface in certain cancers. Metadherin expression is activated by TNF, and metadherin is expressed in many cells types with the highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Metadherin is often over expressed in various cancers including breast, brain, prostate, and melanoma and glioblastoma multiforme. EMD-Millipore’s Anti-metadherin mouse monoclonal, clone 2F11C3 has been tested by western blot against several human cancer cell lysates, by flow cytometry against human HeLa cells, and in human paraffin-embedded human liver tissue.

产品信息
格式	Affinity Purified
控制	K562 cell lysate
演示	Purified mouse monoclonal IgG1 in PBS containing up to 0.1% sodium azide and 50% glycerol.

应用
应用	Detect Metadherin using this mouse monoclonal antibody, Anti-Metadherin Antibody, clone 2F11C3 validated for use in western blotting, IHC & Flow Cytometry.
主要应用	Flow Cytometry Western Blotting Immunohistochemistry
应用说明	Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Metadherin in human breast cancer tissue. Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected Metadherin in HeLa cells Optimal working dilutions must be determined by end user.

生物信息
免疫原品种	Purified protein fragment of Human Metadherin expressed in E. coli.
克隆	2F11C3
宿主	Mouse
同种型	IgG1
品种反应性	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_848927
基因符号	MTDH 3D3 AEG1 LYRIC
纯化方法	Protein G purified
UniProt编号	Q86UE4

产品使用声明
质量保证	Evaluated by Western Blotting in K562 cell lysate. Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Metadherin in 10 µg of K562 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.