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merck millipore,默克密理博,MAB2150,Anti-E-Selectin Antibody, clone P2H3
产品名称：Anti-E-Selectin Antibody, clone P2H3
产品型号：MAB2150
Anti-E-Selectin Antibody, clone P2H3 detects level of E-Selectin & has been published & validated for use in ELISA, FC, IP, IC, IH.
merck millipore,默克密理博,MAB2150,Anti-E-Selectin Antibody, clone P2H3
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IHC, ELISA, FC, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MAB2150 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-E-Selectin Antibody, clone P2H3 Alternate Names - CD62E
- ELAM-1
背景信息 Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface, members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNF alpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NK kappa B dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations. 产品信息 格式 Purified 控制 - Ductal breast cancer or mormal colon
演示 Purified by protein G and stored in 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl containing 0.05% NaN3. 应用 应用 Anti-E-Selectin Antibody, clone P2H3 detects level of E-Selectin & has been published & validated for use in ELISA, FC, IP, IC, IH. 主要应用 - Immunohistochemistry
- ELISA
- Flow Cytometry
- Immunocytochemistry
应用说明 Immunohistochemistry: 1:50 – 1:500; acetone or paraformaldehyde-fixed frozen and formalin-fixed paraffin-embedded tissues; recommend heat-induced epitope retrieval in EDTA, pH 8.0 for paraffin-embedded tissues
Immunofluorescence
Flow Cytometry
Immunoprecipitation
ELISA
Blocking: 10-15µg/mL; inhibits carcinoma and monocyte adhesion to IL-1β activated HUVEC
Optimal working dilutions must be determined by end user.
生物信息 免疫原品种 IL-1(1 activated HUVEC (human) 克隆 P2H3 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Human E-Selectin 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000450.1
Entrez基因汇总 The endothelial leukocyte adhesion molecule-1 is expressed by cytokine-stimulated endothelial cells. It is thought to be responsible for the accumulation of blood leukocytes at sites of inflammation by mediating the adhesion of cells to the vascular lining. It exhibits structural features such as the presence of lectin- and EGF-like domains followed by short consensus repeat (SCR) domains that contain 6 conserved cysteine residues. These proteins are part of the selectin family of cell adhesion molecules. This gene is present in single copy in the human genome and contains 14 exons spanning about 13 kb of DNA. Adhesion molecules participate in the interaction between leukocytes and the endothelium and appear to be involved in the pathogenesis of atherosclerosis. 基因符号 - ELAM1
- SELE
- LECAM2
- ESEL
- ELAM
- ELAM-1
- CD62E
UniProt编号 - P16581
UniProt汇总 FUNCTION: SwissProt: P16581 # Expressed on cytokine induced endothelial cells and mediates their binding to leukocytes. The ligand recognized by ELAM-1 is sialyl-lewis X (alpha(1->3)fucosylated derivatives of polylactosamine that are found at the nonreducing termini of glycolipids).
SIZE: 610 amino acids; 66655 Da
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
SIMILARITY: SwissProt: P16581 ## Belongs to the selectin/LECAM family. & Contains 1 C-type lectin domain. & Contains 1 EGF-like domain. & Contains 6 Sushi (CCP/SCR) domains.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C for up to 12 months from date of receipt.. 包装信息 数量 100 µg
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