merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB16200,Anti-MGMT Antibody, clone MT3.1

  • merck millipore,默克密理博,MAB16200,Anti-MGMT Antibody, clone MT3.1

    产品名称：Anti-MGMT Antibody, clone MT3.1
    产品型号：MAB16200
    Use Anti-MGMT Antibody, clone MT3.1 (mouse monoclonal antibody) validated in FC, ICC,IHC(P), IP, WB to detect MGMT also known as O-6-methylguanine-DNA Methyltransferase, Methylated-DNA--Protein-cystei More>>

    merck millipore,默克密理博,MAB16200,Anti-MGMT Antibody, clone MT3.1

  • 产品介绍
  • merck millipore,默克密理博,MAB16200,Anti-MGMT Antibody, clone MT3.1

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HWB, FC, ICCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB16200
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-MGMT Antibody, clone MT3.1
    Alternate Names
    • Methylated-DNA--Protein-cysteine Methyltransferase
    • O-6-methylguanine-DNA Methyltransferase
    背景信息 O6-Methylguanine-DNA Methyltransferase (MGMT) is a ubiquitous DNA repair protein that removes O6-alkyl-guanine, primarily O6 methylguanine lesions from damaged DNA. It is a major contributor to cellular protection from the mutagenic, carcinogenic, and cytotoxic effects of DNA alkylation. The mechanism of MGMT action is based on the transfer of the alkyl group from the DNA to a unique acceptor cysteine residue in the protein, forming a stable thioether linkage. MGMT is frequently classified as a suicide protein. The repair capacity for O6- methylguanine is dependent on the number of MGMT molecules in the cell. There is a correlation between the occurrence of cancer in various tissues and the lack of the MGMT enzyme. High levels of MGMT result in reduced tumor events and resistance of tumors to alkylating agents.
    产品信息
    格式 Purified
    控制
    • Positive Control: Tonsil tissue, HeLa cells, CEM-CCRF cells.
      Negative Control: TK6 cells.
    演示 Purified mouse monoclonal IgG1 in buffer containing 0.02 M PB, pH 7.6, 0.25 M NaCl containing 0.1% sodium azide.
    应用
    应用 Use Anti-MGMT Antibody, clone MT3.1 (mouse monoclonal antibody) validated in FC, ICC,IHC(P), IP, WB to detect MGMT also known as O-6-methylguanine-DNA Methyltransferase, Methylated-DNA--Protein-cysteine Methyltransferase.
    主要应用
    • Western Blotting
    • Flow Cytometry
    • Immunocytochemistry
    应用说明 Flow Cytometry:
    An independent laboratory used a previous lot on flow cytometry (Gerson, 1996).

    Immunoprecipitation:
    A previous lot of this antibody was used on Immunoprecipitation.

    Immunohistochemistry:
    A previous lot of this antibody was used on paraffin embedded sections.

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 Made against recombinant human MGMT (O-6-methylguanine-DNA methyltransferase) expressed in E. coli.
    克隆 MT3.1
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 MGMT is a 22 kDa DNA repair protein that is present in all normal tissues; however, in a subset of human tumours MGMT is completely absent. Thus, antibody MAB16200 may be helpful in utilizing MGMT as a marker of malignant or pre-malignant cells. Because MGMT repairs the potentially cytotoxic antitumour lesions induced in DNA by certain cancer chemotherapeutic alkylating agents (e.g. BCNU, CCNU, DTIC, procarbazinc, temozolomide), tumours lacking or having low levels of MGMT will predictably be responsive to drug therapy. Conversely, tumours with high MGMT levels will likely be drug resistant. The MGMT antibody may also be used to gauge the effectiveness of MGMT modulators, such as O-6-benzylguanine, which lead to rapid degradation of the inactivated protein.
    同种型 IgG1
    品种反应性 Human
    Species Reactivity Note Useful for detection of MGMT in human tissues, tumours, cells, or xenograft lines. Gives weak cross-reactivity with MGMT from mouse, hamster and rat cells.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_002412.2
    基因符号
    • EC 2.1.1.63
    • MGMT
    纯化方法 Protein A purfied
    UniProt编号
    • P16455
    UniProt汇总 FUNCTION: SwissProt: P16455 # Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
    COFACTOR: Binds 1 zinc ion.
    SIZE: 207 amino acids; 21646 Da
    SUBCELLULAR LOCATION: Nucleus.
    SIMILARITY: SwissProt: P16455 ## Belongs to the MGMT family.
    产品使用声明
    质量保证 Evaluated by Western Blot on Jurkat lysates.

    Western Blot Analysis:
    1:500 dilution of this antibody detected MGMT on 10 µg of Jurkat lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at 2-8ºC from date of receipt.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB16200,Anti-MGMT Antibody, clone MT3.1

上一件merck millipore产品：merck millipore,默克密理博,GF021,Stem Cell Factor Protein, Recombinant human
下一件merck millipore产品：merck millipore,默克密理博,05-799,Anti-Amyloid β (Aβ)x-40 Antibody, clone 11A5-B10


相关产品

merck millipore,默克密理博,03-217,ATG3 (Human) Recombinant Protein merck millipore,默克密理博,03-217,ATG3 (Human) Recombinant Protein

merck millipore,默克密理博,AB5885,Anti-Histamine Antibody merck millipore,默克密理博,AB5885,Anti-Histamine Antibody

merck millipore,默克密理博,ABE289,Anti-trimethyl-Histone H3 (Lys18) Antibody merck millipore,默克密理博,ABE289,Anti-trimethyl-Histone H3 (Lys18) Antibody

merck millipore,默克密理博,CBL419,Anti-Eosinophil Major Basic Protein Antibody, clone BMK13 merck millipore,默克密理博,CBL419,Anti-Eosinophil Major Basic Protein Antibody, clone BMK13

merck millipore,默克密理博,GF021,Stem Cell Factor Protein, Recombinant human merck millipore,默克密理博,GF021,Stem Cell Factor Protein, Recombinant human

merck millipore,默克密理博,05-799,Anti-Amyloid β (Aβ)x-40 Antibody, clone 11A5-B10 merck millipore,默克密理博,05-799,Anti-Amyloid β (Aβ)x-40 Antibody, clone 11A5-B10

merck millipore,默克密理博,14-668,DRAK1 Protein, active, 10 µg merck millipore,默克密理博,14-668,DRAK1 Protein, active, 10 µg

merck millipore,默克密理博,AB136,Anti-Dopa Decarboxylase Antibody merck millipore,默克密理博,AB136,Anti-Dopa Decarboxylase Antibody

merck millipore,默克密理博,14-602M,PI3 Kinase (p110α/p85α) Protein, active, 250 µg merck millipore,默克密理博,14-602M,PI3 Kinase (p110α/p85α) Protein, active, 250 µg

merck millipore,默克密理博,MABN1037,Anti-ROCK2 Antibody, clone EPR7141(B), rabbit monoclonal merck millipore,默克密理博,MABN1037,Anti-ROCK2 Antibody, clone EPR7141(B), rabbit monoclonal


whatman,沃特曼,nalgene,耐洁,merck millipore,默克密理愽,sartorius,赛多利斯,pall,颇尔
thermo fisher,赛默飞世尔,GE,MN,macherey-nagel,advantec,wheaton,brand,普兰德,welch,airtech


上海羽令过滤器材有限公司版权所有 Copyright ©  www.yl-guolv.com  Powered by 羽令集团—信息技术部