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- 体外诊断/OEM材料和试剂
- 产品介绍
描述 产品目录编号 MAB1132-KC 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Human SMAD4/DPC4, clone SMD46 (1mg) KC 概述 This product is intended for use by IVD manufacturers. Alternate Names - DPC4
产品信息 格式 Purified 演示 200 μg/ml antibody purified from ascites fluid by Protein G chromatography. Liquid in 10 mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide. 应用 主要应用 - Western Blotting
应用说明 Immunofluorescence
Western Blotting: Ab 1μg/ml for 2hrs at room temperature
Immunoprecipitation (Use Protein G): Ab 2μg/mg protein lysate
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Purified recombinant DPC4 protein. 克隆 SMD46 宿主 Mouse 特异性 Recognizes a protein of 60kDa, which is identified as DPC4 (also named SMAD4). Antibody is highly specific and shows no cross-reaction with the other closely related members of this family. The family of SMAD molecules comprises essential mediators of transforming growth factor beta (TGF-b) signaling. To date, seven members of this family have been identified, each of which plays a specific and separate role in mediating TGF-beta superfamily gene transcription. At least two different members, Smad2 and Smad4/DPC4, have been implicated in human cancer and appear to have tumor-suppressor functions whose inactivation may play an important role in the histogenesis of pancreatic and colorectal malignancy and possibly other human cancers as well. 同种型 IgG1 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_005359.3
基因符号 - JIP
- SMAD4
- DPC4
- hSMAD4
- MADH4
UniProt编号 - Q13485
UniProt汇总 FUNCTION: SwissProt: Q13485 # Common mediator of signal transduction by TGF-beta (transforming growth factor) superfamily; SMAD4 is the common SMAD (co-SMAD). Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. May act as a tumor suppressor.
SIZE: 552 amino acids; 60439 Da
SUBUNIT: May form trimers with receptor-regulated SMAD (R-SMAD). Interacts with ATF2, COPS5, DACH1, MSG1, SKI and TRIM33. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R- SMAD.
DISEASE: SwissProt: Q13485 # Defects in SMAD4 are a cause of pancreatic carcinoma [MIM:260350]. & Defects in SMAD4 are a cause of juvenile polyposis syndrome (JPS) [MIM:174900]; also known as juvenile intestinal polyposis (JIP). JPS is an autosomal dominant gastrointestinal hamartomatous polyposis syndrome in which patients are at risk for developing gastrointestinal cancers. The lesions are typified by a smooth histological appearance, predominant stroma, cystic spaces and lack of a smooth muscle core. Multiple juvenile polyps usually occur in a number of Mendelian disorders. Sometimes, these polyps occur without associated features as in JPS; here, polyps tend to occur in the large bowel and are associated with an increased risk of colon and other gastrointestinal cancers. & Defects in SMAD4 are a cause of juvenile polyposis/hereditary hemorrhagic telangiectasia syndrome (JP/HHT) [MIM:175050]. JP/HHT syndrome phenotype consists of the coexistence of juvenile polyposis (JIP) and hereditary hemorrhagic telangiectasia (HHT) [MIM:187300] in a single individual. JIP and HHT are autosomal dominant disorders with distinct and non- overlapping clinical features. The former, an inherited gastrointestinal malignancy predisposition, is caused by mutations in SMAD4 or BMPR1A, and the latter is a vascular malformation disorder caused by mutations in ENG or ACVRL1. All four genes encode proteins involved in the transforming-growth-factor- signaling pathway. Although there are reports of patients and families with phenotypes of both disorders combined, the genetic aetiology of this association is unknown. & Defects in SMAD4 may be a cause of colorectal cancer (CRC) [MIM:114500].
SIMILARITY: SwissProt: Q13485 ## Belongs to the dwarfin/SMAD family. & Contains 1 MH1 (MAD homology 1) domain. & Contains 1 MH2 (MAD homology 2) domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months. 包装信息 数量 Bulk packaging. Please inquire.
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