merck millipore,默克密理愽
  • merck millipore,默克密理博,CBL221,Anti-RNA Polymerase II Antibody, clone ARNA-3

  • merck millipore,默克密理博,CBL221,Anti-RNA Polymerase II Antibody, clone ARNA-3

    产品名称：Anti-RNA Polymerase II Antibody, clone ARNA-3
    产品型号：CBL221
    Detect RNA Polymerase II with Anti-RNA Polymerase II Antibody, clone ARNA-3 (Mouse Monoclonal Antibody), that has been shown to work in WB & IHC.

    merck millipore,默克密理博,CBL221,Anti-RNA Polymerase II Antibody, clone ARNA-3

  • 产品介绍
  • merck millipore,默克密理博,CBL221,Anti-RNA Polymerase II Antibody, clone ARNA-3

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Insect, HIHC, WBMAscitesMonoclonal Antibody
    描述
    产品目录编号 CBL221
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-RNA Polymerase II Antibody, clone ARNA-3
    产品信息
    格式 Ascites
    控制
    • Positive for Drosophila chromosomes and cell lines PTK-2, HeLa and Hep2.
    演示 Supplied as tissue culture supernatant concentrate containing 0.09% sodium azide.
    应用
    应用 Detect RNA Polymerase II with Anti-RNA Polymerase II Antibody, clone ARNA-3 (Mouse Monoclonal Antibody), that has been shown to work in WB & IHC.
    主要应用
    • Immunohistochemistry
    • Western Blotting
    应用说明 Western blot
    Immunohistochemistry: 1:100; Frozen tissue; fixation recommendation - incubate tissue with 2% paraformaldehyde in PBS for 10 min, wash in PBS for 5 min, incubate in 0.2% Triton X-100 in PBS for 5 min, wash in PBS for 5 min. Incubate with antibody one hour.
    Optimal working dilutions must be determined by the end user.
    生物信息
    免疫原品种 Purified RNA Polymerase II
    表位 amino acids 794-822
    克隆 ARNA-3
    宿主 Mouse
    特异性 ARNA-3 is excellent for detection of transcription activity, e.g. puffs in polytene chromosomes. Reacts with RNA polymerase II (175 kDa subunit of RNAP II) in biological materials. Reactive polypeptides after SDS-PAGE: 200 kDa band of nonphosphorylated and 240 kDa band of phosphorylated Pol IIA.
    同种型 IgG1
    品种反应性 InsectHuman
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_057088.2
    Entrez基因汇总 The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmanns Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
    基因符号
    • Keratin-3
    • KRT3
    • CK3
    • K3
    • CK-3
    • Cytokeratin-3
    UniProt编号
    • P12035
    UniProt汇总 SIZE: 629 amino acids; 64504 Da
    SUBUNIT: Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.
    TISSUE SPECIFICITY: Cornea specific.
    DISEASE: SwissProt: P12035 # Defects in KRT3 are a cause of Meesmann corneal dystrophy (MCD) [MIM:122100]. MCD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.
    SIMILARITY: SwissProt: P12035 ## Belongs to the intermediate filament family.
    MISCELLANEOUS: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain at 2°-8°C for up to 12 months from date of receipt.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,CBL221,Anti-RNA Polymerase II Antibody, clone ARNA-3

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