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merck millipore,默克密理博,AP1064,PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb
产品名称：PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb
产品型号：AP1064
This PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb is validated for use in Immunoblotting, Immunocytochemistry for the detection of PDH-E1α (pSer³⁰⁰).
merck millipore,默克密理博,AP1064,PhosphoDetect™ Anti-PDH-E1α (pSer³⁰⁰) Rabbit pAb
- 产品介绍
重要规格表
宿主 Rb 描述 概述 Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser300 in HEK293 cells. 产品目录编号 AP1064 品牌系列 Calbiochem®
同义词 Anti-Pyruvate Dehydogenase pSer³⁰⁰ Rabbit pAb 应用数据 
Detection of human PDH-E1α, phosphorylated at Ser300, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (Lane 1) or treated with dichloroacetate (Lane 2). Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (0.5 µg/ml). Detection: chemiluminescence.
Detection of human PDH-E1α, phosphorylated at Ser300, by immunocytochemistry. Samples: HEK293 cells left untreated (top panels) or treated with dichloroacetate (lower panels). Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer300) Rabbit pAb (Cat. No. AP1064) (1µg/ml). Detection: fluorescence with DAPI (blue, nuclei) and Alexa Fluor 488® dye-Phalloidin (green, actin filaments) counterstains.参考 参考 Rardin M.J., et. al. 2009. Anal. Biochem. 2, 157.
Seifert, F., et al. 2007. Biochemistry 21, 6277.
Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
Patel, M.S. and Korotchkina, L.G. 2006 Biochem. Soc. Trans. 34, 217.
Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.产品信息 形式 Liquid 配方 In 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4. 负控制 HEK293 cells treated with dichloroacetate 正控制 HEK293 cells 防腐剂 ≤0.1% sodium azide 应用 主要应用 Immunoblotting (Western Blotting)
Immunocytochemistry
生物信息 免疫原 a synthetic phosphopeptide corresponding to amino acids surrounding the Ser³⁰⁰ phosphorylation site of human PDH-E1α 免疫原品种 Human 宿主 Rabbit 品种 human 浓缩标签 Please refer to vial label for lot-specific concentration
储存和货运信息 货运代码 Blue Ice Only
毒性 Standard Handling
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-20°C).
上一件merck millipore产品：merck millipore,默克密理博,34103,EDTA, Tetrasodium Tetrahydrate Salt - CAS 13235-36-4 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,ECM582,QCM Haptotaxis Cell Migration Assay -Collagen I, 24-well, colorimetric
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