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merck millipore,默克密理博,ABC27,Anti-ICAD (DFF) (cleaved form) Antibody
产品名称：Anti-ICAD (DFF) (cleaved form) Antibody
产品型号：ABC27
Detect ICAD (DFF) (cleaved form) using this Anti-ICAD (DFF) (cleaved form) Antibody validated for use in WB & IC.
merck millipore,默克密理博,ABC27,Anti-ICAD (DFF) (cleaved form) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABC27 描述 Anti-ICAD (DFF) (cleaved form) Antibody Alternate Names - Inhibitor of CAD, DNA degradation of chromosomal DNA, DNase
- ICAD
- DFF-45
- DNA fragmentation factor subunit alpha
- DNA fragmentation factor 45 kDa subunit
背景信息 Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These cell death signals cause the degradation of chromosomal DNA by activated DNase. A human DNA fragmentation factor (DFF) was identified recently which was cleaved by caspase-3 during apoptosis (Liu et al. 1997). The mouse homologue of this human DFF was identified as a DNase inhibitor designated ICAD (Enari et al. 1998; Sakahira et al. 1998). Upon cleavage of DFF/ICAD, a caspase activated deoxyribonuclease (CAD) is released, activated and eventually causes the degradation of DNA in the nuclei (Enari et al. 1998; Sakahira et al. 1998). Therefore, the cleavage of CAD inhibitor molecule DFF/ICAD, which causes DNase activation and DNA degradation, is identified as a hallmark of apoptotic cell death (Wylile et al. 1998). 产品信息 格式 Affinity Purified 控制 - Untreated and staurosporin-treated HeLa cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect ICAD (DFF) (cleaved form) using this Anti-ICAD (DFF) (cleaved form) Antibody validated for use in WB & IC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected cleaved ICAD (DFF) in staurosporin-treated HeLa cells. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the endogenous, cleaved form of human ICAD (DFF). 宿主 Rabbit 特异性 This antibody recognizes the endogenous, cleaved form of ICAD (DFF). 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_004392
Entrez基因汇总 Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. 基因符号 - DFF-45
- DFF1
- DFFA
- DFF45
- H13
纯化方法 Affinity Purified UniProt编号 - O00273
UniProt汇总 FUNCTION: Inhibitor of the caspase-activated DNase (DFF40).
SUBUNIT STRUCTURE: Heterodimer of DFFA and DFFB.
SUBCELLULAR LOCALIZATION: Cytoplasm.产品使用声明 质量保证 Evaluated by Western Blot in HeLa cell lysate untreated and staurosporin-treated.
Western Blot Analysis: A 1:500 dilution of this antibody detected cleaved ICAD (DFF) in untreated and staurosporin-treated HeLa cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,8310-OP,OmniPur 20X SSC Buffer Liquid Concentrate - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,203408,BLEOCIN™ Antibiotic, Streptomyces verticillus, Cell Culture-Tested - CAS 55658-47-4 - Calbiochem
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