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merck millipore,默克密理博,3244,LIGHT DIAGNOSTICS CMV pp65 Antigenemia Antibody Set, ~125 tests, included in kit #3247
产品名称：LIGHT DIAGNOSTICS CMV pp65 Antigenemia Antibody Set, ~125 tests, included in kit #3247
产品型号：3244
merck millipore,默克密理博,3244,LIGHT DIAGNOSTICS CMV pp65 Antigenemia Antibody Set, ~125 tests, included in kit #3247
- 产品介绍
- merck millipore,默克密理博,3244,LIGHT DIAGNOSTICS CMV pp65 Antigenemia Antibody Set, ~125 tests, included in kit #3247
重要规格表
主要应用 检测方法 ICC Fluorescent 描述 产品目录编号 3244 品牌系列 Chemicon®
商名 - Chemicon
- LIGHT DIAGNOSTICS
描述 LIGHT DIAGNOSTICS CMV pp65 Antigenemia Antibody Set, ~125 tests, included in kit #3247 概述 INTENDED USE:
The Light Diagnostics Cytomegalovirus (CMV) pp65 Antigenemia indirect immunofluorescence assay (IFA) is intended for the qualitative identification of lower matrix protein pp65 of CMV in isolated peripheral blood leukocytes.
TEST PRINCIPLE:
The Light Diagnostics CMV pp65 Antigenemia assay utilizes an indirect immunofluorescence technique for identifying the lower matrix protein pp65 of human CMV in cytospin preparations of peripheral blood leukocytes. The blend of monoclonal antibodies provided will bind to CMV pp65 antigen present in formalin fixed leukocytes. Unbound monoclonal antibody is removed by washing with phosphate buffered saline (PBS). Fluorescein isothiocyanate (FITC) conjugated antibody will bind to the antigen-antibody complex. Unbound conjugate is removed by washing with PBS. FITC exhibits an apple green fluorescence when excited by ultraviolet light allowing visualization of the complex by fluorescence microscopy. Nuclear fluorescence indicates a positive specimen. Uninfected cells stain dull red due to the presence of Evans blue in the FITC conjugated antibody reagent.
SUMMARY AND EXPLANATION:
CMV is a member of the family Herpesviridae. Synthesis of viral DNA and assembly of capsids occur in the nucleus where infective progeny are released by budding through the nuclear envelope. Also characteristic of herpes viruses, CMV undergoes periods of latency and reactivation.
CMV is species specific but has been isolated from many animal species. The first human CMV was isolated in 1956 from embryonic fibroblasts of adenoidal tissue. The terminal morphology of CMV infected permissive cells is that of a large cell with a prominent intranuclear inclusion (Cowdry Type A or "owl eye" cell). Such cells were identified in tissues of fatally infected infants which gave rise to the name "cytomegalic inclusion disease (CID)".
Humans are believed to be the only reservoir of human CMV and postnatal infections are acquired by close contact with individuals shedding virus. Transmission may be through saliva, urine, cervical and vaginal secretions, semen, breast milk, tears, feces, and blood. Infection rates vary with geographic location and socioeconomic conditions.
CMV infection has been detected in newborn infants and is the most commonly identified cause of congenital infection. Those infants that develop symptoms may exhibit severe disease with jaundice, hepatosplenomegaly, petechiae, and central nervous system abnormalities. The risk of infection is probably the same throughout pregnancy; CID occurs most often in fetuses infected during the first half of gestation. CMV may be transmitted to about 50% of the fetuses after primary maternal infection and about 10% of these will be clinically affected. Many congenitally infected infants appear normal at birth but subsequently develop neurologic sequelae. The route of transmission of CMV from mother to fetus has not been well elucidated. It is possible that the spread is hematogenous through cord blood or placental tissue and amniotic cells.
During the past decade an increasing population of immunosuppressed individuals has resulted in a resurgence of CMV as a major pathogen. Induced immunosuppression has occurred more frequently via chemotherapy and transplant regimens. CMV infection is common in patients receiving renal, bone marrow, heart, lung, and liver transplants. The spread of acquired immunodeficiency syndrome (AIDS) is also related to the CMV resurgence. CMV is the most common viral opportunistic infection in AIDS patients and may be a cofactor in the pathogenesis of the AIDS virus. CMV infection in AIDS has been implicated in pneumonitis, colitis, retinitis, and dementia.
Detection of CMV in blood leukocytes is closely associated with the clinical manifestations of CMV disease and is useful in the diagnosis of CMV infection. Rapid diagnosis of CMV disease may prevent delay in treatment using antiviral drugs such as ganciclovir and foscarnet. The Light Diagnostics CMV pp65 Antigenemia assay is a rapid, sensitive method for detection of CMV in isolated leukocytes.产品信息 部件 - Mounting Fluid - (Catalog No. 5013) One dropper vial containing 10 mL of Tris buffered glycerin, a fluorescence enhancer and less than 0.1% sodium azide.
- CMV pp65 Monoclonal Antibody - (Catalog No. 5097R) One dropper vial containing 5 mL of a blend of monoclonal antibodies against CMV pp65 antigen, protein stabilizer, 0.05% Tween 20, and less than 0.1% sodium azide.
- Anti Mouse IgG / FITC Conjugate - (Catalog No. 5024) One dropper vial containing 10 mL of FITC labeled anti-mouse IgG antibody, 0.02% Evans blue, protein stabilizer and less than 0.1% sodium azide.
检测方法 Fluorescent 应用 主要应用 - Immunocytochemistry
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
包装信息 数量 5 mL
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