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重要规格表
检测方法 Colorimetric 描述 概述 A non-radioactive competitive immunoassay kit for the rapid quantitative measurement of cAMP. No neutralization, extraction, drying, or reconstitution of samples is required. Samples are treated with 0.1N HCl to lyse the cells, stop endogenous phosphodiesterase activity, and stabilize the released cAMP, which can then be analyzed directly in the microtiter plate. 产品目录编号 116811 品牌系列 Calbiochem®
参考 参考 Chard, T. In: An Introduction to Radioimmunoassay and Related Techniques. 1990. 4th Ed., Elsevier, Amsterdam.
NCCLS Evaluation Protocols, SC1, 1989, NCCLS, Villanova, PA, 19085.
Tijssen, P. In: Practice and Theory of Enzyme Immunoassays. 1985. Elsevier, Amsterdam.
Chabardes, D., et al. 1980. J. Clin. Invest. 65, 439.
Grill, V., and Cerasi, E. 1974. J. Biol. Chem. 249, 41961.
Szentivanyi, A. 1968. J. Allergy 42, 203.
Haynes, R.C. 1958. J. Biol. Chem. 233, 1220.产品信息 检测方法 Colorimetric 形式 100 Tests 格式 96-well format 试剂盒包含 One Antibody Coated 96-Well Plate, cAMP Direct Conjugate, Anti-cAMP, cAMP Standard, 0.1 N HCl, Neutralizing Reagent, Wash Buffer, Color Substrate, Stop Solution, Triethylamine, Acetic Anhydride, a Plate Sealer, and a user protocol. 应用 应用参考 Gettys, T.W., et al. 1991. J. Biol. Chem. 266, 15949. Gettys, T.W., et al. 1990. 2nd Messengers & Phosphoprot. 13, 37. Yamamoto, I., and Tsuji, J. 1981. Immunopharm. 3, 53. Collins, W.P., and Hennam, J.F. 1976. In: Molecular Aspects of Medicine Vol. 1, (Baum, H. and Cergeley, J., eds.) p. 3, Pergamon, England. Fausto, H., and Butcher, F.R. 1976. Biochim. Biophys. Acta 428, 702. Farmer, R.W., et al. 1975. Anal. Biochem. 64, 455. Steiner, A.L. 1974. Methods Enzymol. 38, 96. 生物信息 化验范围 0.78 - 200 pmol/ml (non-acetylated), 0.078-20 pmol/ml (acetylated) 化验时间 4.5 h 样品类型 Serum, cell, and tissue samples 理化信息 灵敏度 390 fmol/ml (non-acetylated), 37 fmol/ml (acetylated) 安全信息 R相 R: 11-20/21-28-32-35-50-53
Highly flammable.
Harmful by inhalation and in contact with skin.
Very toxic if swallowed.
Contact with acids liberates very toxic gas.
Causes severe burns.
Very toxic to aquatic organisms.
May cause long-term adverse effects in the aquatic environment.S相 S: 26-3-16-27-28-29-36/37/39-45
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Keep in a cool place.
Keep away from sources of ignition - No Smoking.
Take off immediately all contaminated clothing.
Do not empty into drains.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).产品使用声明 预定用途 The Calbiochem® cAMP Direct Immunoassay Kit, Colorimetric is a competitive immunoassay for the quantitative assay of cAMP in cell lysates, tissue extracts, and serum/plasma. 储存和货运信息 货运代码 Blue Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项： Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 +2°C to +8°C
存储条件 Upon arrival store the cAMP Direct Conjugate and the Cyclic AMP Standard at -20°C and the remaining components of the kit at 4°C. 无需冷冻 No
补充信息 试剂盒包含 One Antibody Coated 96-Well Plate, cAMP Direct Conjugate, Anti-cAMP, cAMP Standard, 0.1 N HCl, Neutralizing Reagent, Wash Buffer, Color Substrate, Stop Solution, Triethylamine, Acetic Anhydride, a Plate Sealer, and a user protocol.
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