- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, M WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 05-710 品牌系列 Upstate
商名 - Upstate
描述 Anti-NG2 Antibody, clone 132.38 产品信息 格式 Purified 控制 - Brain tissue
演示 Protein A Purified immunoglobulin in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl with 0.05% sodium azide as a preservative. 应用 应用 Anti-NG2 Antibody, clone 132.38 detects level of NG2 & has been published & validated for use in IH & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Cross-reactivity expected with mouse. 生物信息 免疫原品种 HEK293 cells expressing a truncated integral membrane form of NG2 consisting of amino acids 1592-2222 克隆 132.38 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes NG2. 同种型 IgG1 品种反应性 RatMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001897
Entrez基因汇总 A human melanoma-associated chondroitin sulfate proteoglycan plays a role in stabilizing cell-substratum interactions during early events of melanoma cell spreading on endothelial basement membranes. CSPG4 represents an integral membrane chondroitin sulfate proteoglycan expressed by human malignant melanoma cells. 基因符号 - MEL-CSPG
- CSPG4
- MCSP
- HMW-MAA
- MCSPG
- MSK16
- NG2
纯化方法 Protein G purfied UniProt编号 - Q6UVK1
UniProt汇总 FUNCTION: SwissProt: Q6UVK1 # Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.
SIZE: 2322 amino acids; 250497 Da
SUBUNIT: Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA. Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2 (By similarity). Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16.
SUBCELLULAR LOCATION: Apical cell membrane; Single-pass type I membrane protein (By similarity). Cell projection, lamellipodium (By similarity). Note=Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. Localizes to the plasma membrane of oligodendrocytes (By similarity).
TISSUE SPECIFICITY: Detected only in malignant melanoma cells.
PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation (By similarity). Involved in interaction with MMP16 and ITGA4. & Phosphorylation by PRKCA regulates its subcellular location and function in cell motility (By similarity).
SIMILARITY: SwissProt: Q6UVK1 ## Contains 15 CSPG (NG2) repeats. & Contains 2 laminin G-like domains.
MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells.产品使用声明 质量保证 routinely evaluated by immunoblot on whole rat brain preparation 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,07-2263,Anti-WNK4 Antibody
下一件merck millipore产品：merck millipore,默克密理博,SF-2517,MAP3K1; Human, Cyanine 3 RNA Detection Probe,SmartFlare
相关产品
merck millipore,默克密理博,MABC148,Anti-Casper/c-FLIP Antibody, clone 17B12.1
merck millipore,默克密理博,GF094,Vascular Endothelial Growth Factor, Recombinant human, 165aa isoform
merck millipore,默克密理博,ABN368,Anti-Spastin Antibody
merck millipore,默克密理博,SF-2058,FANCG; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,07-2263,Anti-WNK4 Antibody
merck millipore,默克密理博,SF-2517,MAP3K1; Human, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,CBL589F,Anti-Integrin αIIb Subunit Antibody, clone PM6/248, FITC conjugated
merck millipore,默克密理博,70598,pETBlue-1 AccepTor™ Vector Kit - Novagen
merck millipore,默克密理博,MABC632,Anti-MAP3K5 Antibody, clone 2E4